Western Blot:Article Title: HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HOXC6 (Immunoblotting and ChIP) Invitrogen Cat# PA5-68513; RRID: AB_2689510 HOXC6 (Immunohistochemistry) Invitrogen Cat# PA5-41479; RRID: AB_2609572 ACTINB Cell Signaling Cat#4970; RRID: AB_2223172 pAKT (Ser473) Cell Signaling Cat#4060; RRID: AB_2315049 tAKT Cell Signaling Cat#9272; RRID: AB_329827 pERK1/2 Cell Signaling Cat#4376; RRID: AB_331772 Total-ERK1/2 Cell Signaling Cat#4695; RRID: AB_390779 V5 Cell Signaling Cat#13202; RRID: AB_2687461 MSK1 (Immunoblotting) Cell Signaling Cat# 3489; RRID: AB_2285349 MSK1 (Immunohistochemistry) ABCAM Cat# Ab99412 Phospho-p70 S6 Kinase (Thr389) Cell Signaling Cat# 9205; RRID: AB_330944 p70 S6 Kinase Cell Signaling Cat# 9202; RRID: AB_331676 PPP2R2B Invitrogen Cat# PA5-72539; RRID: AB_2718393 Cleaved Caspase-3 Cell Signaling Cat# 9664; RRID: AB_2070042 DDX17 (Immunoblotting) Santa Cruz Cat# sc-398168 DDX17 (Immunoprecipitation) Novus Biologicals Cat# NBP2-87261 DDX17 (ChIP) Proteintech Cat# 19910-1-AP; RRID: AB_10667004 Anti-Phosphoserine Antibody Sigma-Aldrich Cat# AB1603; RRID:AB_390205 Phospho-IGF-I Receptor b (Tyr980) Cell Signaling Cat# 4568; RRID: AB_2122279 IGF-I Receptor b Cell Signaling Cat# 3027; RRID:AB_2122378 Phospho-EphA2 (Ser897) Cell Signaling Cat# 6347; RRID: AB_11220420 EphA2 Cell Signaling Cat# 6997; RRID: AB_10827743 Phospho-Insulin Receptor b (Tyr1345) Cell Signaling Cat# 3026; RRID: AB_2127116 Insulin Receptor b Cell Signaling Cat# 3025; RRID: AB_2280448 C/EBPB (Immunoblotting) Cell Signaling Cat# 43095 C/EBPB (ChIP) Invitrogen Cat# PA5-27244; RRID: AB_2544720 YBX1 (Immunoblotting) Cell Signaling Cat# 4202; RRID: AB_1950384 YBX1 (ChIP) Invitrogen Cat# MA5-42819; RRID: AB_2911960 MCL1 Cell Signaling Cat# 94296; RRID: AB_2722740 Biological samples Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# HPan-Ade150CS-02 Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# PA601 Chemicals, peptides, and recombinant proteins DMEM GIBCO Cat# 11965-092 RPMI GIBCO Cat# 11875-093 Fetal Bovine Serum GIBCO Cat# 10437-028 Trypsin-EDTA GIBCO Cat# 25200-056 Penicillin-Streptomycin GIBCO Cat# 15140-122 Effectene Transfection Reagent QIAGEN Cat# 301427 Agarose, Low gelling Sigma-Aldrich Cat# A9045 Matrigel Invasion Chambers BD Biosciences Cat# 354483 (Continued on next page) e1 Cell Reports Medicine 4, 101285, November 21, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER VECTASHIELD Hardset Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1500 XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate Perkin Elmer Cat# 122799 Matrigel Basement Membrane Matrix Corning Cat# 356237 Advanced DMEM:F12 Thermo Fisher Cat# 12634-028 HEPES Thermo Fisher Cat# 15630-080 L-Glutamine ATCC ATCC 30-2214 B-27 Supplement Thermo Fisher Cat# 17504-044 Noggin R&D Systems Cat# 6057-NG EGF R&D Systems Cat# 236-EG FGF-10 R&D Systems Cat# 345-FG Gastrin R&D Systems Cat# 3006 A83-01 R&D Systems Cat# 2939 GlutaMAX Thermo Fisher Cat# 35050-079 Nicotinamide Sigma-Aldrich Cat# N0636 N-acetyl cysteine Sigma-Aldrich Cat# A9165 Geltrex Matrigel Thermo Fisher Cat# A1413202 TrypLE Thermo Fisher Cat # 12604-013 Trametinib Selleckchem Cat# S2673 Buparlisib (BKM120) Selleckchem Cat# S2247 Rapamycin Selleckchem Cat# S1039 Everolimus Selleckchem Cat# S1120 SB-747651A Tocris Cat# 4630 Y-27632 (hydrochloride) Cayman Chemical Cat# 10005583 BMS-754807 Selleckchem Cat# S1124 Critical commercial assays Protein Synthesis Assay Kit Cayman Chemical Cat# 601100 Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit R&D Systems Cat# ARY001B Click-iTTM Plus OPP Alexa FluorTM 594 Protein Synthesis Assay Kit Invitrogen Cat# C10457 Annexin V staining Flow Cytometry kit BD Pharmingen Cat# 559763 CellTiter-Glo 3D Cell Viability Assay Kit Promega Cat# G9681 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Cat# 9002 Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Kit Invitrogen Cat# C10635 MSK1 Kinase Enzyme System Promega Cat# V5093 Deposited Data RNA-Seq performed with HOXC6 KD PANC1 cells This paper GEO: GSE146944 RNA-Seq performed with MSK1 KD PANC1 cells This paper GEO: GSE150222 RNA-Seq performed with MSK1 KD AsPC1 cells This paper GEO: GSE150223 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in PANC1 cells This paper PRIDE: PXD041238 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in AsPC1 cells This paper PRIDE: PXD041239 (Continued on next page) Cell Reports Medicine 4, 101285, November 21, 2023 e2
Chromatin Immunoprecipitation:Article Title: HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HOXC6 (Immunoblotting and ChIP) Invitrogen Cat# PA5-68513; RRID: AB_2689510 HOXC6 (Immunohistochemistry) Invitrogen Cat# PA5-41479; RRID: AB_2609572 ACTINB Cell Signaling Cat#4970; RRID: AB_2223172 pAKT (Ser473) Cell Signaling Cat#4060; RRID: AB_2315049 tAKT Cell Signaling Cat#9272; RRID: AB_329827 pERK1/2 Cell Signaling Cat#4376; RRID: AB_331772 Total-ERK1/2 Cell Signaling Cat#4695; RRID: AB_390779 V5 Cell Signaling Cat#13202; RRID: AB_2687461 MSK1 (Immunoblotting) Cell Signaling Cat# 3489; RRID: AB_2285349 MSK1 (Immunohistochemistry) ABCAM Cat# Ab99412 Phospho-p70 S6 Kinase (Thr389) Cell Signaling Cat# 9205; RRID: AB_330944 p70 S6 Kinase Cell Signaling Cat# 9202; RRID: AB_331676 PPP2R2B Invitrogen Cat# PA5-72539; RRID: AB_2718393 Cleaved Caspase-3 Cell Signaling Cat# 9664; RRID: AB_2070042 DDX17 (Immunoblotting) Santa Cruz Cat# sc-398168 DDX17 (Immunoprecipitation) Novus Biologicals Cat# NBP2-87261 DDX17 (ChIP) Proteintech Cat# 19910-1-AP; RRID: AB_10667004 Anti-Phosphoserine Antibody Sigma-Aldrich Cat# AB1603; RRID:AB_390205 Phospho-IGF-I Receptor b (Tyr980) Cell Signaling Cat# 4568; RRID: AB_2122279 IGF-I Receptor b Cell Signaling Cat# 3027; RRID:AB_2122378 Phospho-EphA2 (Ser897) Cell Signaling Cat# 6347; RRID: AB_11220420 EphA2 Cell Signaling Cat# 6997; RRID: AB_10827743 Phospho-Insulin Receptor b (Tyr1345) Cell Signaling Cat# 3026; RRID: AB_2127116 Insulin Receptor b Cell Signaling Cat# 3025; RRID: AB_2280448 C/EBPB (Immunoblotting) Cell Signaling Cat# 43095 C/EBPB (ChIP) Invitrogen Cat# PA5-27244; RRID: AB_2544720 YBX1 (Immunoblotting) Cell Signaling Cat# 4202; RRID: AB_1950384 YBX1 (ChIP) Invitrogen Cat# MA5-42819; RRID: AB_2911960 MCL1 Cell Signaling Cat# 94296; RRID: AB_2722740 Biological samples Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# HPan-Ade150CS-02 Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# PA601 Chemicals, peptides, and recombinant proteins DMEM GIBCO Cat# 11965-092 RPMI GIBCO Cat# 11875-093 Fetal Bovine Serum GIBCO Cat# 10437-028 Trypsin-EDTA GIBCO Cat# 25200-056 Penicillin-Streptomycin GIBCO Cat# 15140-122 Effectene Transfection Reagent QIAGEN Cat# 301427 Agarose, Low gelling Sigma-Aldrich Cat# A9045 Matrigel Invasion Chambers BD Biosciences Cat# 354483 (Continued on next page) e1 Cell Reports Medicine 4, 101285, November 21, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER VECTASHIELD Hardset Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1500 XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate Perkin Elmer Cat# 122799 Matrigel Basement Membrane Matrix Corning Cat# 356237 Advanced DMEM:F12 Thermo Fisher Cat# 12634-028 HEPES Thermo Fisher Cat# 15630-080 L-Glutamine ATCC ATCC 30-2214 B-27 Supplement Thermo Fisher Cat# 17504-044 Noggin R&D Systems Cat# 6057-NG EGF R&D Systems Cat# 236-EG FGF-10 R&D Systems Cat# 345-FG Gastrin R&D Systems Cat# 3006 A83-01 R&D Systems Cat# 2939 GlutaMAX Thermo Fisher Cat# 35050-079 Nicotinamide Sigma-Aldrich Cat# N0636 N-acetyl cysteine Sigma-Aldrich Cat# A9165 Geltrex Matrigel Thermo Fisher Cat# A1413202 TrypLE Thermo Fisher Cat # 12604-013 Trametinib Selleckchem Cat# S2673 Buparlisib (BKM120) Selleckchem Cat# S2247 Rapamycin Selleckchem Cat# S1039 Everolimus Selleckchem Cat# S1120 SB-747651A Tocris Cat# 4630 Y-27632 (hydrochloride) Cayman Chemical Cat# 10005583 BMS-754807 Selleckchem Cat# S1124 Critical commercial assays Protein Synthesis Assay Kit Cayman Chemical Cat# 601100 Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit R&D Systems Cat# ARY001B Click-iTTM Plus OPP Alexa FluorTM 594 Protein Synthesis Assay Kit Invitrogen Cat# C10457 Annexin V staining Flow Cytometry kit BD Pharmingen Cat# 559763 CellTiter-Glo 3D Cell Viability Assay Kit Promega Cat# G9681 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Cat# 9002 Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Kit Invitrogen Cat# C10635 MSK1 Kinase Enzyme System Promega Cat# V5093 Deposited Data RNA-Seq performed with HOXC6 KD PANC1 cells This paper GEO: GSE146944 RNA-Seq performed with MSK1 KD PANC1 cells This paper GEO: GSE150222 RNA-Seq performed with MSK1 KD AsPC1 cells This paper GEO: GSE150223 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in PANC1 cells This paper PRIDE: PXD041238 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in AsPC1 cells This paper PRIDE: PXD041239 (Continued on next page) Cell Reports Medicine 4, 101285, November 21, 2023 e2
Immunohistochemistry:Article Title: HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HOXC6 (Immunoblotting and ChIP) Invitrogen Cat# PA5-68513; RRID: AB_2689510 HOXC6 (Immunohistochemistry) Invitrogen Cat# PA5-41479; RRID: AB_2609572 ACTINB Cell Signaling Cat#4970; RRID: AB_2223172 pAKT (Ser473) Cell Signaling Cat#4060; RRID: AB_2315049 tAKT Cell Signaling Cat#9272; RRID: AB_329827 pERK1/2 Cell Signaling Cat#4376; RRID: AB_331772 Total-ERK1/2 Cell Signaling Cat#4695; RRID: AB_390779 V5 Cell Signaling Cat#13202; RRID: AB_2687461 MSK1 (Immunoblotting) Cell Signaling Cat# 3489; RRID: AB_2285349 MSK1 (Immunohistochemistry) ABCAM Cat# Ab99412 Phospho-p70 S6 Kinase (Thr389) Cell Signaling Cat# 9205; RRID: AB_330944 p70 S6 Kinase Cell Signaling Cat# 9202; RRID: AB_331676 PPP2R2B Invitrogen Cat# PA5-72539; RRID: AB_2718393 Cleaved Caspase-3 Cell Signaling Cat# 9664; RRID: AB_2070042 DDX17 (Immunoblotting) Santa Cruz Cat# sc-398168 DDX17 (Immunoprecipitation) Novus Biologicals Cat# NBP2-87261 DDX17 (ChIP) Proteintech Cat# 19910-1-AP; RRID: AB_10667004 Anti-Phosphoserine Antibody Sigma-Aldrich Cat# AB1603; RRID:AB_390205 Phospho-IGF-I Receptor b (Tyr980) Cell Signaling Cat# 4568; RRID: AB_2122279 IGF-I Receptor b Cell Signaling Cat# 3027; RRID:AB_2122378 Phospho-EphA2 (Ser897) Cell Signaling Cat# 6347; RRID: AB_11220420 EphA2 Cell Signaling Cat# 6997; RRID: AB_10827743 Phospho-Insulin Receptor b (Tyr1345) Cell Signaling Cat# 3026; RRID: AB_2127116 Insulin Receptor b Cell Signaling Cat# 3025; RRID: AB_2280448 C/EBPB (Immunoblotting) Cell Signaling Cat# 43095 C/EBPB (ChIP) Invitrogen Cat# PA5-27244; RRID: AB_2544720 YBX1 (Immunoblotting) Cell Signaling Cat# 4202; RRID: AB_1950384 YBX1 (ChIP) Invitrogen Cat# MA5-42819; RRID: AB_2911960 MCL1 Cell Signaling Cat# 94296; RRID: AB_2722740 Biological samples Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# HPan-Ade150CS-02 Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# PA601 Chemicals, peptides, and recombinant proteins DMEM GIBCO Cat# 11965-092 RPMI GIBCO Cat# 11875-093 Fetal Bovine Serum GIBCO Cat# 10437-028 Trypsin-EDTA GIBCO Cat# 25200-056 Penicillin-Streptomycin GIBCO Cat# 15140-122 Effectene Transfection Reagent QIAGEN Cat# 301427 Agarose, Low gelling Sigma-Aldrich Cat# A9045 Matrigel Invasion Chambers BD Biosciences Cat# 354483 (Continued on next page) e1 Cell Reports Medicine 4, 101285, November 21, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER VECTASHIELD Hardset Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1500 XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate Perkin Elmer Cat# 122799 Matrigel Basement Membrane Matrix Corning Cat# 356237 Advanced DMEM:F12 Thermo Fisher Cat# 12634-028 HEPES Thermo Fisher Cat# 15630-080 L-Glutamine ATCC ATCC 30-2214 B-27 Supplement Thermo Fisher Cat# 17504-044 Noggin R&D Systems Cat# 6057-NG EGF R&D Systems Cat# 236-EG FGF-10 R&D Systems Cat# 345-FG Gastrin R&D Systems Cat# 3006 A83-01 R&D Systems Cat# 2939 GlutaMAX Thermo Fisher Cat# 35050-079 Nicotinamide Sigma-Aldrich Cat# N0636 N-acetyl cysteine Sigma-Aldrich Cat# A9165 Geltrex Matrigel Thermo Fisher Cat# A1413202 TrypLE Thermo Fisher Cat # 12604-013 Trametinib Selleckchem Cat# S2673 Buparlisib (BKM120) Selleckchem Cat# S2247 Rapamycin Selleckchem Cat# S1039 Everolimus Selleckchem Cat# S1120 SB-747651A Tocris Cat# 4630 Y-27632 (hydrochloride) Cayman Chemical Cat# 10005583 BMS-754807 Selleckchem Cat# S1124 Critical commercial assays Protein Synthesis Assay Kit Cayman Chemical Cat# 601100 Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit R&D Systems Cat# ARY001B Click-iTTM Plus OPP Alexa FluorTM 594 Protein Synthesis Assay Kit Invitrogen Cat# C10457 Annexin V staining Flow Cytometry kit BD Pharmingen Cat# 559763 CellTiter-Glo 3D Cell Viability Assay Kit Promega Cat# G9681 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Cat# 9002 Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Kit Invitrogen Cat# C10635 MSK1 Kinase Enzyme System Promega Cat# V5093 Deposited Data RNA-Seq performed with HOXC6 KD PANC1 cells This paper GEO: GSE146944 RNA-Seq performed with MSK1 KD PANC1 cells This paper GEO: GSE150222 RNA-Seq performed with MSK1 KD AsPC1 cells This paper GEO: GSE150223 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in PANC1 cells This paper PRIDE: PXD041238 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in AsPC1 cells This paper PRIDE: PXD041239 (Continued on next page) Cell Reports Medicine 4, 101285, November 21, 2023 e2
Immunoprecipitation:Article Title: HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HOXC6 (Immunoblotting and ChIP) Invitrogen Cat# PA5-68513; RRID: AB_2689510 HOXC6 (Immunohistochemistry) Invitrogen Cat# PA5-41479; RRID: AB_2609572 ACTINB Cell Signaling Cat#4970; RRID: AB_2223172 pAKT (Ser473) Cell Signaling Cat#4060; RRID: AB_2315049 tAKT Cell Signaling Cat#9272; RRID: AB_329827 pERK1/2 Cell Signaling Cat#4376; RRID: AB_331772 Total-ERK1/2 Cell Signaling Cat#4695; RRID: AB_390779 V5 Cell Signaling Cat#13202; RRID: AB_2687461 MSK1 (Immunoblotting) Cell Signaling Cat# 3489; RRID: AB_2285349 MSK1 (Immunohistochemistry) ABCAM Cat# Ab99412 Phospho-p70 S6 Kinase (Thr389) Cell Signaling Cat# 9205; RRID: AB_330944 p70 S6 Kinase Cell Signaling Cat# 9202; RRID: AB_331676 PPP2R2B Invitrogen Cat# PA5-72539; RRID: AB_2718393 Cleaved Caspase-3 Cell Signaling Cat# 9664; RRID: AB_2070042 DDX17 (Immunoblotting) Santa Cruz Cat# sc-398168 DDX17 (Immunoprecipitation) Novus Biologicals Cat# NBP2-87261 DDX17 (ChIP) Proteintech Cat# 19910-1-AP; RRID: AB_10667004 Anti-Phosphoserine Antibody Sigma-Aldrich Cat# AB1603; RRID:AB_390205 Phospho-IGF-I Receptor b (Tyr980) Cell Signaling Cat# 4568; RRID: AB_2122279 IGF-I Receptor b Cell Signaling Cat# 3027; RRID:AB_2122378 Phospho-EphA2 (Ser897) Cell Signaling Cat# 6347; RRID: AB_11220420 EphA2 Cell Signaling Cat# 6997; RRID: AB_10827743 Phospho-Insulin Receptor b (Tyr1345) Cell Signaling Cat# 3026; RRID: AB_2127116 Insulin Receptor b Cell Signaling Cat# 3025; RRID: AB_2280448 C/EBPB (Immunoblotting) Cell Signaling Cat# 43095 C/EBPB (ChIP) Invitrogen Cat# PA5-27244; RRID: AB_2544720 YBX1 (Immunoblotting) Cell Signaling Cat# 4202; RRID: AB_1950384 YBX1 (ChIP) Invitrogen Cat# MA5-42819; RRID: AB_2911960 MCL1 Cell Signaling Cat# 94296; RRID: AB_2722740 Biological samples Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# HPan-Ade150CS-02 Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# PA601 Chemicals, peptides, and recombinant proteins DMEM GIBCO Cat# 11965-092 RPMI GIBCO Cat# 11875-093 Fetal Bovine Serum GIBCO Cat# 10437-028 Trypsin-EDTA GIBCO Cat# 25200-056 Penicillin-Streptomycin GIBCO Cat# 15140-122 Effectene Transfection Reagent QIAGEN Cat# 301427 Agarose, Low gelling Sigma-Aldrich Cat# A9045 Matrigel Invasion Chambers BD Biosciences Cat# 354483 (Continued on next page) e1 Cell Reports Medicine 4, 101285, November 21, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER VECTASHIELD Hardset Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1500 XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate Perkin Elmer Cat# 122799 Matrigel Basement Membrane Matrix Corning Cat# 356237 Advanced DMEM:F12 Thermo Fisher Cat# 12634-028 HEPES Thermo Fisher Cat# 15630-080 L-Glutamine ATCC ATCC 30-2214 B-27 Supplement Thermo Fisher Cat# 17504-044 Noggin R&D Systems Cat# 6057-NG EGF R&D Systems Cat# 236-EG FGF-10 R&D Systems Cat# 345-FG Gastrin R&D Systems Cat# 3006 A83-01 R&D Systems Cat# 2939 GlutaMAX Thermo Fisher Cat# 35050-079 Nicotinamide Sigma-Aldrich Cat# N0636 N-acetyl cysteine Sigma-Aldrich Cat# A9165 Geltrex Matrigel Thermo Fisher Cat# A1413202 TrypLE Thermo Fisher Cat # 12604-013 Trametinib Selleckchem Cat# S2673 Buparlisib (BKM120) Selleckchem Cat# S2247 Rapamycin Selleckchem Cat# S1039 Everolimus Selleckchem Cat# S1120 SB-747651A Tocris Cat# 4630 Y-27632 (hydrochloride) Cayman Chemical Cat# 10005583 BMS-754807 Selleckchem Cat# S1124 Critical commercial assays Protein Synthesis Assay Kit Cayman Chemical Cat# 601100 Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit R&D Systems Cat# ARY001B Click-iTTM Plus OPP Alexa FluorTM 594 Protein Synthesis Assay Kit Invitrogen Cat# C10457 Annexin V staining Flow Cytometry kit BD Pharmingen Cat# 559763 CellTiter-Glo 3D Cell Viability Assay Kit Promega Cat# G9681 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Cat# 9002 Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Kit Invitrogen Cat# C10635 MSK1 Kinase Enzyme System Promega Cat# V5093 Deposited Data RNA-Seq performed with HOXC6 KD PANC1 cells This paper GEO: GSE146944 RNA-Seq performed with MSK1 KD PANC1 cells This paper GEO: GSE150222 RNA-Seq performed with MSK1 KD AsPC1 cells This paper GEO: GSE150223 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in PANC1 cells This paper PRIDE: PXD041238 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in AsPC1 cells This paper PRIDE: PXD041239 (Continued on next page) Cell Reports Medicine 4, 101285, November 21, 2023 e2
Microarray:Article Title: HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HOXC6 (Immunoblotting and ChIP) Invitrogen Cat# PA5-68513; RRID: AB_2689510 HOXC6 (Immunohistochemistry) Invitrogen Cat# PA5-41479; RRID: AB_2609572 ACTINB Cell Signaling Cat#4970; RRID: AB_2223172 pAKT (Ser473) Cell Signaling Cat#4060; RRID: AB_2315049 tAKT Cell Signaling Cat#9272; RRID: AB_329827 pERK1/2 Cell Signaling Cat#4376; RRID: AB_331772 Total-ERK1/2 Cell Signaling Cat#4695; RRID: AB_390779 V5 Cell Signaling Cat#13202; RRID: AB_2687461 MSK1 (Immunoblotting) Cell Signaling Cat# 3489; RRID: AB_2285349 MSK1 (Immunohistochemistry) ABCAM Cat# Ab99412 Phospho-p70 S6 Kinase (Thr389) Cell Signaling Cat# 9205; RRID: AB_330944 p70 S6 Kinase Cell Signaling Cat# 9202; RRID: AB_331676 PPP2R2B Invitrogen Cat# PA5-72539; RRID: AB_2718393 Cleaved Caspase-3 Cell Signaling Cat# 9664; RRID: AB_2070042 DDX17 (Immunoblotting) Santa Cruz Cat# sc-398168 DDX17 (Immunoprecipitation) Novus Biologicals Cat# NBP2-87261 DDX17 (ChIP) Proteintech Cat# 19910-1-AP; RRID: AB_10667004 Anti-Phosphoserine Antibody Sigma-Aldrich Cat# AB1603; RRID:AB_390205 Phospho-IGF-I Receptor b (Tyr980) Cell Signaling Cat# 4568; RRID: AB_2122279 IGF-I Receptor b Cell Signaling Cat# 3027; RRID:AB_2122378 Phospho-EphA2 (Ser897) Cell Signaling Cat# 6347; RRID: AB_11220420 EphA2 Cell Signaling Cat# 6997; RRID: AB_10827743 Phospho-Insulin Receptor b (Tyr1345) Cell Signaling Cat# 3026; RRID: AB_2127116 Insulin Receptor b Cell Signaling Cat# 3025; RRID: AB_2280448 C/EBPB (Immunoblotting) Cell Signaling Cat# 43095 C/EBPB (ChIP) Invitrogen Cat# PA5-27244; RRID: AB_2544720 YBX1 (Immunoblotting) Cell Signaling Cat# 4202; RRID: AB_1950384 YBX1 (ChIP) Invitrogen Cat# MA5-42819; RRID: AB_2911960 MCL1 Cell Signaling Cat# 94296; RRID: AB_2722740 Biological samples Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# HPan-Ade150CS-02 Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# PA601 Chemicals, peptides, and recombinant proteins DMEM GIBCO Cat# 11965-092 RPMI GIBCO Cat# 11875-093 Fetal Bovine Serum GIBCO Cat# 10437-028 Trypsin-EDTA GIBCO Cat# 25200-056 Penicillin-Streptomycin GIBCO Cat# 15140-122 Effectene Transfection Reagent QIAGEN Cat# 301427 Agarose, Low gelling Sigma-Aldrich Cat# A9045 Matrigel Invasion Chambers BD Biosciences Cat# 354483 (Continued on next page) e1 Cell Reports Medicine 4, 101285, November 21, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER VECTASHIELD Hardset Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1500 XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate Perkin Elmer Cat# 122799 Matrigel Basement Membrane Matrix Corning Cat# 356237 Advanced DMEM:F12 Thermo Fisher Cat# 12634-028 HEPES Thermo Fisher Cat# 15630-080 L-Glutamine ATCC ATCC 30-2214 B-27 Supplement Thermo Fisher Cat# 17504-044 Noggin R&D Systems Cat# 6057-NG EGF R&D Systems Cat# 236-EG FGF-10 R&D Systems Cat# 345-FG Gastrin R&D Systems Cat# 3006 A83-01 R&D Systems Cat# 2939 GlutaMAX Thermo Fisher Cat# 35050-079 Nicotinamide Sigma-Aldrich Cat# N0636 N-acetyl cysteine Sigma-Aldrich Cat# A9165 Geltrex Matrigel Thermo Fisher Cat# A1413202 TrypLE Thermo Fisher Cat # 12604-013 Trametinib Selleckchem Cat# S2673 Buparlisib (BKM120) Selleckchem Cat# S2247 Rapamycin Selleckchem Cat# S1039 Everolimus Selleckchem Cat# S1120 SB-747651A Tocris Cat# 4630 Y-27632 (hydrochloride) Cayman Chemical Cat# 10005583 BMS-754807 Selleckchem Cat# S1124 Critical commercial assays Protein Synthesis Assay Kit Cayman Chemical Cat# 601100 Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit R&D Systems Cat# ARY001B Click-iTTM Plus OPP Alexa FluorTM 594 Protein Synthesis Assay Kit Invitrogen Cat# C10457 Annexin V staining Flow Cytometry kit BD Pharmingen Cat# 559763 CellTiter-Glo 3D Cell Viability Assay Kit Promega Cat# G9681 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Cat# 9002 Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Kit Invitrogen Cat# C10635 MSK1 Kinase Enzyme System Promega Cat# V5093 Deposited Data RNA-Seq performed with HOXC6 KD PANC1 cells This paper GEO: GSE146944 RNA-Seq performed with MSK1 KD PANC1 cells This paper GEO: GSE150222 RNA-Seq performed with MSK1 KD AsPC1 cells This paper GEO: GSE150223 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in PANC1 cells This paper PRIDE: PXD041238 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in AsPC1 cells This paper PRIDE: PXD041239 (Continued on next page) Cell Reports Medicine 4, 101285, November 21, 2023 e2
Recombinant:Article Title: HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HOXC6 (Immunoblotting and ChIP) Invitrogen Cat# PA5-68513; RRID: AB_2689510 HOXC6 (Immunohistochemistry) Invitrogen Cat# PA5-41479; RRID: AB_2609572 ACTINB Cell Signaling Cat#4970; RRID: AB_2223172 pAKT (Ser473) Cell Signaling Cat#4060; RRID: AB_2315049 tAKT Cell Signaling Cat#9272; RRID: AB_329827 pERK1/2 Cell Signaling Cat#4376; RRID: AB_331772 Total-ERK1/2 Cell Signaling Cat#4695; RRID: AB_390779 V5 Cell Signaling Cat#13202; RRID: AB_2687461 MSK1 (Immunoblotting) Cell Signaling Cat# 3489; RRID: AB_2285349 MSK1 (Immunohistochemistry) ABCAM Cat# Ab99412 Phospho-p70 S6 Kinase (Thr389) Cell Signaling Cat# 9205; RRID: AB_330944 p70 S6 Kinase Cell Signaling Cat# 9202; RRID: AB_331676 PPP2R2B Invitrogen Cat# PA5-72539; RRID: AB_2718393 Cleaved Caspase-3 Cell Signaling Cat# 9664; RRID: AB_2070042 DDX17 (Immunoblotting) Santa Cruz Cat# sc-398168 DDX17 (Immunoprecipitation) Novus Biologicals Cat# NBP2-87261 DDX17 (ChIP) Proteintech Cat# 19910-1-AP; RRID: AB_10667004 Anti-Phosphoserine Antibody Sigma-Aldrich Cat# AB1603; RRID:AB_390205 Phospho-IGF-I Receptor b (Tyr980) Cell Signaling Cat# 4568; RRID: AB_2122279 IGF-I Receptor b Cell Signaling Cat# 3027; RRID:AB_2122378 Phospho-EphA2 (Ser897) Cell Signaling Cat# 6347; RRID: AB_11220420 EphA2 Cell Signaling Cat# 6997; RRID: AB_10827743 Phospho-Insulin Receptor b (Tyr1345) Cell Signaling Cat# 3026; RRID: AB_2127116 Insulin Receptor b Cell Signaling Cat# 3025; RRID: AB_2280448 C/EBPB (Immunoblotting) Cell Signaling Cat# 43095 C/EBPB (ChIP) Invitrogen Cat# PA5-27244; RRID: AB_2544720 YBX1 (Immunoblotting) Cell Signaling Cat# 4202; RRID: AB_1950384 YBX1 (ChIP) Invitrogen Cat# MA5-42819; RRID: AB_2911960 MCL1 Cell Signaling Cat# 94296; RRID: AB_2722740 Biological samples Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# HPan-Ade150CS-02 Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# PA601 Chemicals, peptides, and recombinant proteins DMEM GIBCO Cat# 11965-092 RPMI GIBCO Cat# 11875-093 Fetal Bovine Serum GIBCO Cat# 10437-028 Trypsin-EDTA GIBCO Cat# 25200-056 Penicillin-Streptomycin GIBCO Cat# 15140-122 Effectene Transfection Reagent QIAGEN Cat# 301427 Agarose, Low gelling Sigma-Aldrich Cat# A9045 Matrigel Invasion Chambers BD Biosciences Cat# 354483 (Continued on next page) e1 Cell Reports Medicine 4, 101285, November 21, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER VECTASHIELD Hardset Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1500 XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate Perkin Elmer Cat# 122799 Matrigel Basement Membrane Matrix Corning Cat# 356237 Advanced DMEM:F12 Thermo Fisher Cat# 12634-028 HEPES Thermo Fisher Cat# 15630-080 L-Glutamine ATCC ATCC 30-2214 B-27 Supplement Thermo Fisher Cat# 17504-044 Noggin R&D Systems Cat# 6057-NG EGF R&D Systems Cat# 236-EG FGF-10 R&D Systems Cat# 345-FG Gastrin R&D Systems Cat# 3006 A83-01 R&D Systems Cat# 2939 GlutaMAX Thermo Fisher Cat# 35050-079 Nicotinamide Sigma-Aldrich Cat# N0636 N-acetyl cysteine Sigma-Aldrich Cat# A9165 Geltrex Matrigel Thermo Fisher Cat# A1413202 TrypLE Thermo Fisher Cat # 12604-013 Trametinib Selleckchem Cat# S2673 Buparlisib (BKM120) Selleckchem Cat# S2247 Rapamycin Selleckchem Cat# S1039 Everolimus Selleckchem Cat# S1120 SB-747651A Tocris Cat# 4630 Y-27632 (hydrochloride) Cayman Chemical Cat# 10005583 BMS-754807 Selleckchem Cat# S1124 Critical commercial assays Protein Synthesis Assay Kit Cayman Chemical Cat# 601100 Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit R&D Systems Cat# ARY001B Click-iTTM Plus OPP Alexa FluorTM 594 Protein Synthesis Assay Kit Invitrogen Cat# C10457 Annexin V staining Flow Cytometry kit BD Pharmingen Cat# 559763 CellTiter-Glo 3D Cell Viability Assay Kit Promega Cat# G9681 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Cat# 9002 Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Kit Invitrogen Cat# C10635 MSK1 Kinase Enzyme System Promega Cat# V5093 Deposited Data RNA-Seq performed with HOXC6 KD PANC1 cells This paper GEO: GSE146944 RNA-Seq performed with MSK1 KD PANC1 cells This paper GEO: GSE150222 RNA-Seq performed with MSK1 KD AsPC1 cells This paper GEO: GSE150223 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in PANC1 cells This paper PRIDE: PXD041238 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in AsPC1 cells This paper PRIDE: PXD041239 (Continued on next page) Cell Reports Medicine 4, 101285, November 21, 2023 e2
Transfection:Article Title: HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HOXC6 (Immunoblotting and ChIP) Invitrogen Cat# PA5-68513; RRID: AB_2689510 HOXC6 (Immunohistochemistry) Invitrogen Cat# PA5-41479; RRID: AB_2609572 ACTINB Cell Signaling Cat#4970; RRID: AB_2223172 pAKT (Ser473) Cell Signaling Cat#4060; RRID: AB_2315049 tAKT Cell Signaling Cat#9272; RRID: AB_329827 pERK1/2 Cell Signaling Cat#4376; RRID: AB_331772 Total-ERK1/2 Cell Signaling Cat#4695; RRID: AB_390779 V5 Cell Signaling Cat#13202; RRID: AB_2687461 MSK1 (Immunoblotting) Cell Signaling Cat# 3489; RRID: AB_2285349 MSK1 (Immunohistochemistry) ABCAM Cat# Ab99412 Phospho-p70 S6 Kinase (Thr389) Cell Signaling Cat# 9205; RRID: AB_330944 p70 S6 Kinase Cell Signaling Cat# 9202; RRID: AB_331676 PPP2R2B Invitrogen Cat# PA5-72539; RRID: AB_2718393 Cleaved Caspase-3 Cell Signaling Cat# 9664; RRID: AB_2070042 DDX17 (Immunoblotting) Santa Cruz Cat# sc-398168 DDX17 (Immunoprecipitation) Novus Biologicals Cat# NBP2-87261 DDX17 (ChIP) Proteintech Cat# 19910-1-AP; RRID: AB_10667004 Anti-Phosphoserine Antibody Sigma-Aldrich Cat# AB1603; RRID:AB_390205 Phospho-IGF-I Receptor b (Tyr980) Cell Signaling Cat# 4568; RRID: AB_2122279 IGF-I Receptor b Cell Signaling Cat# 3027; RRID:AB_2122378 Phospho-EphA2 (Ser897) Cell Signaling Cat# 6347; RRID: AB_11220420 EphA2 Cell Signaling Cat# 6997; RRID: AB_10827743 Phospho-Insulin Receptor b (Tyr1345) Cell Signaling Cat# 3026; RRID: AB_2127116 Insulin Receptor b Cell Signaling Cat# 3025; RRID: AB_2280448 C/EBPB (Immunoblotting) Cell Signaling Cat# 43095 C/EBPB (ChIP) Invitrogen Cat# PA5-27244; RRID: AB_2544720 YBX1 (Immunoblotting) Cell Signaling Cat# 4202; RRID: AB_1950384 YBX1 (ChIP) Invitrogen Cat# MA5-42819; RRID: AB_2911960 MCL1 Cell Signaling Cat# 94296; RRID: AB_2722740 Biological samples Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# HPan-Ade150CS-02 Tissue microarray slide (PDAC and matched normal pancreas tissue) US Biomax, Inc. Cat# PA601 Chemicals, peptides, and recombinant proteins DMEM GIBCO Cat# 11965-092 RPMI GIBCO Cat# 11875-093 Fetal Bovine Serum GIBCO Cat# 10437-028 Trypsin-EDTA GIBCO Cat# 25200-056 Penicillin-Streptomycin GIBCO Cat# 15140-122 Effectene Transfection Reagent QIAGEN Cat# 301427 Agarose, Low gelling Sigma-Aldrich Cat# A9045 Matrigel Invasion Chambers BD Biosciences Cat# 354483 (Continued on next page) e1 Cell Reports Medicine 4, 101285, November 21, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER VECTASHIELD Hardset Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1500 XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate Perkin Elmer Cat# 122799 Matrigel Basement Membrane Matrix Corning Cat# 356237 Advanced DMEM:F12 Thermo Fisher Cat# 12634-028 HEPES Thermo Fisher Cat# 15630-080 L-Glutamine ATCC ATCC 30-2214 B-27 Supplement Thermo Fisher Cat# 17504-044 Noggin R&D Systems Cat# 6057-NG EGF R&D Systems Cat# 236-EG FGF-10 R&D Systems Cat# 345-FG Gastrin R&D Systems Cat# 3006 A83-01 R&D Systems Cat# 2939 GlutaMAX Thermo Fisher Cat# 35050-079 Nicotinamide Sigma-Aldrich Cat# N0636 N-acetyl cysteine Sigma-Aldrich Cat# A9165 Geltrex Matrigel Thermo Fisher Cat# A1413202 TrypLE Thermo Fisher Cat # 12604-013 Trametinib Selleckchem Cat# S2673 Buparlisib (BKM120) Selleckchem Cat# S2247 Rapamycin Selleckchem Cat# S1039 Everolimus Selleckchem Cat# S1120 SB-747651A Tocris Cat# 4630 Y-27632 (hydrochloride) Cayman Chemical Cat# 10005583 BMS-754807 Selleckchem Cat# S1124 Critical commercial assays Protein Synthesis Assay Kit Cayman Chemical Cat# 601100 Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit R&D Systems Cat# ARY001B Click-iTTM Plus OPP Alexa FluorTM 594 Protein Synthesis Assay Kit Invitrogen Cat# C10457 Annexin V staining Flow Cytometry kit BD Pharmingen Cat# 559763 CellTiter-Glo 3D Cell Viability Assay Kit Promega Cat# G9681 SimpleChIP Enzymatic Chromatin IP Kit Cell Signaling Cat# 9002 Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Kit Invitrogen Cat# C10635 MSK1 Kinase Enzyme System Promega Cat# V5093 Deposited Data RNA-Seq performed with HOXC6 KD PANC1 cells This paper GEO: GSE146944 RNA-Seq performed with MSK1 KD PANC1 cells This paper GEO: GSE150222 RNA-Seq performed with MSK1 KD AsPC1 cells This paper GEO: GSE150223 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in PANC1 cells This paper PRIDE: PXD041238 Complete list of identified phosphorylated proteins in TMT10-based phosphoproteomics analysis using SB-747651A in AsPC1 cells This paper PRIDE: PXD041239 (Continued on next page) Cell Reports Medicine 4, 101285, November 21, 2023 e2
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![Schematic illustration of the differential effects of finerenone vs eplerenone on G protein-coupled receptor-kinase 5-dependent repression of the cardiac mineralocorticoid receptor. Finerenone, unlike eplerenone, stimulates G protein-coupled receptor-kinase (GRK)-5 to phosphorylate the mineralocorticoid receptor (MR). The two main (putative) GRK5 phosphorylation sites on the human MR protein, Ser601 and Ser843, are highlighted, along with their functional impacts for the MR (pSer601 blocks nuclear translocation; pSer843 suppresses Aldo-induced transcriptional activity)[ , ]. GPCR: G protein-coupled receptor; NTD: N-terminal domain; DBD: DNA-binding domain; LBD: Ligand-binding domain; pSer: Phosphoserine. See text for more details and for all other molecular acronyms' descriptions.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8278/pmc09048278/pmc09048278__WJC-14-220-g005.jpg)